PUBBLICAZIONI

2020

 

Magrelli Federica M, Merra A., Pellegrini G. Surgery Versus ATMPs: An Example From Ophthalmology. Front Bioeng Biotechnol. 2020 Jun 10;8:440. doi: 10.3389/fbioe.2020.00440. eCollection 2020.

 

De Rosa L, Latella MC, Secone Seconetti A, Cattelani C, Bauer JW, Bondanza S, De Luca M. Toward Combined Cell and Gene Therapy for Genodermatoses. Cold Spring Harb Perspect Biol. 2020 May 1;12(5):a035667. doi: 10.1101/cshperspect.a035667. Epub 2019 Oct 25.

 

2019

 

Deng SX, Borderie V, Chan CC, Dana R, Figueiredo FC, Gomes JAP, Pellegrini G, Shimmura S, Kruse FE. Reply. Cornea. 2019 Dec;38(12):e56-e57. doi: 10.1097/ICO.0000000000002145.

 

Vukasovic A, Asnaghi MA, Kostesic P, Quasnichka H, Cozzolino C, Pusic M, Hails L, Trainor N, Krause C, Figallo E, Filardo G, Kon E, Wixmerten A, Maticic D, Pellegrini G, Kafienah W, Hudetz D, Smith T, Martin I, Ivkovic A, Wendt D. Bioreactor-manufactured cartilage grafts repair acute and chronic osteochondral defects in large animal studies. Cell Prolif. 2019 Nov;52(6):e12653. doi: 10.1111/cpr.12653. Epub 2019 Sep 6. 

 

Barbalinardo M, Di Giosia M, Polishchuk I, Magnabosco G, Fermani S, Biscarini F, Calvaresi M, Zerbetto F, Pellegrini G, Falini G, Pokroy B, Valle F. Retinoic acid/calcite micro-carriers inserted in fibrin scaffolds modulate neuronal cell differentiation. J Mater Chem B. 2019 Oct 14;7(38):5808-5813. doi: 10.1039/c9tb01148j. Epub 2019 Sep 5.

 

Sceberras V, Attico E, Bianchi E, Galaverni G, Melonari M, Corradini F, Fantacci M, Ribbene A, Losi L, Balò S, Lazzeri M, Trombetta C, Rizzo M, Manfredini R, Barbagli G, Pellegrini G. Preclinical study for treatment of hypospadias by advanced therapy medicinal products.. World J Urol. 2019 Jul 9. doi: 10.1007/s00345-019-02864-x. [Epub ahead of print]

 

De Luca M, Aiuti A, Cossu G, Parmar M, Pellegrini G, Robey PG. Advances in stem cell research and therapeutic development. Nat Cell Biol. 2019 Jul;21(7):801-811. doi: 10.1038/s41556-019-0344-z. Epub 2019 Jun 17

 

De Rosa L, Secone Seconetti A, De Santis G, Pellacani G, Hirsch T, Rothoeft T, Teig N, Pellegrini G, Bauer JW, De Luca M. Laminin 332-Dependent YAP Dysregulation Depletes Epidermal Stem Cells in Junctional Epidermolysis Bullosa. Cell Rep. 2019 May 14;27(7):2036-2049.e6. doi: 10.1016/j.celrep.2019.04.055.

 

De Pace B, Fiorentini C, Ciardo S1 Chester J, Kaleci S, Veltri T, De Luca M, Pellacani G. Inherited epidermolysis bullosa: description of clinical and subclinical morphological features with optical coherence tomography. J Eur Acad Dermatol Venereol. 2019 Mar;33(3):e120-e123. doi: 10.1111/jdv.15304. Epub 2018 Nov 15.

 

Deng SX, Borderie V, Chan CC, Dana R, Figueiredo FC, Gomes JAP, Pellegrini G, Shimmura S, Kruse FE; and The International Limbal Stem Cell Deficiency Working Group. Global Consensus on Definition, Classification, Diagnosis, and Staging of Limbal Stem Cell Deficiency. Cornea. 2019 Mar;38(3):364-375. doi: 10.1097/ICO.0000000000001820.

 

Rossignoli F, Grisendi G, Spano C, Golinelli G, Recchia A, Rovesti G, Orsi G, Veronesi E, Horwitz EM, Dominici M. Inducible Caspase9-mediated suicide gene for MSC-based cancer gene therapy. Cancer Gene Ther. 2019 Feb;26(1-2):11-16. doi: 10.1038/s41417-018-0034-1. Epub 2018 Jun 29.

 

Recchia A. AAV-CRISPR Persistence in the Eye of the Beholder. Mol Ther. 2019 Jan 2;27(1):12-14. doi: 10.1016/j.ymthe.2018.12.007. Epub 2018 Dec 14.

 

Benati D, Marigo V, Recchia A. CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo. Methods Mol Biol. 2019;1834:59-74. doi: 10.1007/978-1-4939-8669-9_4.

2018

Pellegrini G, De Luca M. Living with Keratinocytes. Stem Cell Reports. 2018 Nov 13;11(5):1026-1033. doi: 10.1016/j.stemcr.2018.10.005.

De Pace B, Fiorentini C, Ciardo S, Chester J, Kaleci S, Veltri T, De Luca M, Pellacani G. Inherited epidermolysis bullosa: description of clinical and subclinical morphological features with optical coherence tomography. J Eur Acad Dermatol Venereol. 2018 Oct 25. doi: 10.1111/jdv.15304. [Epub ahead of print]

Lincoln V, Cogan J, Hou Y, Hirsch M, Hao M, Alexeev V, De Luca M, De Rosa L, Bauer JW, Woodley DT, Chen M. Gentamicin induces LAMB3 nonsense mutation readthrough and restores functional laminin 332 in junctional epidermolysis bullosa. Proc Natl Acad Sci U S A. 2018 Jun 26. pii: 201803154. doi: 10.1073/pnas.1803154115. [Epub ahead of print]

Stern JH, Tian Y, Funderburgh J, Pellegrini G, Zhang K, Goldberg JL, Ali RR, Young M, Xie Y, Temple S. Regenerating Eye Tissues to Preserve and Restore Vision. Cell Stem Cell. 2018 Jun 1;22(6):834-849. doi: 10.1016/j.stem.2018.05.013

Pellegrini G, Ardigò D, Milazzo G, Iotti G, Guatelli P, Pelosi D, De Luca M. Navigating Market Authorization: The Path Holoclar Took to Become the First Stem Cell Product Approved in the European Union. Stem Cells Transl Med. 2018 Jan;7(1):146-154. doi: 10.1002/sctm.17-0003. Epub 2017 Dec 27

2017

Hirsch T, Rothoeft T, Teig N, Bauer JW, Pellegrini G, De Rosa L, Scaglione D, Reichelt J, Klausegger A, Kneisz D, Romano O, Secone Seconetti A, Contin R, Enzo E, Jurman I, Carulli S, Jacobsen F, Luecke T, Lehnhardt M, Fischer M, Kueckelhaus M, Quaglino D, Morgante M, Bicciato S, Bondanza S, De Luca M. Regeneration of the entire human epidermis using transgenic stem cells. Nature. 2017 Nov 16;551(7680):327-332. doi: 10.1038/nature24487. Epub 2017 Nov 8.

Sipp D, Caulfield T, Kaye J, Barfoot J, Blackburn C, Chan S, De Luca M, Kent A, McCabe C, Munsie M, Sleeboom-Faulkner M, Sugarman J, van Zimmeren E, Zarzeczny A, Rasko JEJ. Marketing of unproven stem cell-based interventions: A call to action. Sci Transl Med. 2017 Jul 5;9(397). pii: eaag0426. doi: 10.1126/scitranslmed.aag0426

Rama P, Ferrari G, Pellegrini G. Cultivated limbal epithelial transplantation. Curr Opin Ophthalmol. 2017 Jul;28(4):387-389. doi: 10.1097/ICU.0000000000000382

Latella MC, Cocchiarella F, De Rosa L, Turchiano G, Gonçalves MA, Larcher F, De Luca M, Recchia A. Correction of recessive dystrophic epidermolysis bullosa by transposon-mediated integration of COL7A1 in transplantable patient-derived primary keratinocytes. J Invest Dermatol. 2017 Apr; 137(4):836-44. Epub 2016 Dec 24

Bauer JW, Koller J, Murauer EM, De Rosa L, Enzo E, Carulli S, Bondanza S, Recchia A, Muss W, Diem A, Mayr E, Schlager P, Gratz IK, Pellegrini G, De Luca M. Closure of a large chronic wound through transplantation of gene-corrected epidermal stem cells. J Invest Dermatol.  2017 Mar;137(3):778-781. doi: 10.1016/j.jid.2016.10.038.  Epub 2016 Nov 10

2016

Barbagli G, Pellegrini G, Corradini F, Montorsi F, Sansalone S, Butnaru D, Lazzeri M. One-stage Penile Urethroplasty Using Oral Mucosal Graft and Glue. Eur Urol. 2016 Dec;70(6):1069-1075. doi: 10.1016/j.eururo.2016.04.025. Epub 2016 May 6.

Milazzo G, Ardigò D, Toschi M, Matuska S, Rama P, De Luca M, Pellegrini G. Holoclar: first of its kind in more ways than one. Cell Gene Therapy Insights 2016 July 6;2(2):183-197. doi:10.18609/cgti.2016.023

Cossu G, Buccione R, De Luca M. A discussion on cell therapy in Manchester. Stem Cell Res. 2016 May;16(3):614-6. doi: 10.1016/j.scr.2016.03.005. Epub 2016 Mar 21.

Pellegrini G, Lambiase A, Macaluso C, Pocobelli A, Deng S, Cavallini GM, Esteki R, Rama P, From discovery to approval of an advanced therapy medicinal product-containing stem cells, in the EU. Regen Med. 2016 Jun;11(4):407-20. doi: 10.2217/rme-2015-0051. Epub 2016 Apr 19.

Corradini F, Zattoni M, Barbagli G, Bianchi G, Giovanardi M, Serafini C, Genna V, Ribbene A, Balò S, Fidanza F, Lazzeri M, De Luca M, Pellegrini G. Comparative Assessment Of Cultures From Oral And Urethral Stem Cells For Urethral Regeneration. Curr Stem Cell Res Ther. 2016; 11(8):643-65. doi: 10.2174/1574888X10666150902094644 Epub 2015 Sep 1

2015

Murauer EM, Koller U, Pellegrini G, De Luca M, Bauer JW, Advances in Gene/Cell Therapy in Epidermolysis Bullosa, Keio J Med. 2015 Jun 25;64(2):21-5. doi: 10.2302/kjm.2014-0013-RE. Epub 2015 Jun 6

Tenedini E, Artuso L, Bernardis I, Artusi V, Percesepe A, De Rosa L, Contin R, Manfredini R, Pellecani G, Giannetti A, Pagani J, De Luca M, Tagliafico E. Amplicon-based NGS: an effective approach for the molecular diagnosis of Epidermolysis Bullosa. Br J Dermatol. 2015  Sep;173(3):731-8. doi: 10.1111/bjd.13858. Epub 2015 Jul 29.

2014

Ianiro A, Giosia MD, Fermani S, Samorì C, Barbalinardo M, Valle F, Pellegrini G, Biscarini F, Zerbetto F, Calvaresi M, Falini G. Customizing properties of β-chitin in squid pen (gladius) by chemical treatments. Mar Drugs. 2014 Dec 15;12(12):5979-92. doi: 10.3390/md12125979.

Cavallini GM, Pellegrini G, Volante V, Ducange P, De Maria M, Torlai G, Benatti C, Forlini M, Chemical injury treated with autologous limbal epithelial stem cell transplantation and subconjunctival bevacizumab, Clinical Ophthalmology, 2014 August 30;8:1671–1673 doi: 10.2147/OPTH.S65298. eCollection 2014.

Pellegrini G, De Luca M, Eyes on the prize: limbal stem cells and corneal restoration, Cell Stem Cell. 2014 Aug 7;15(2):121-2. doi: 10.1016/j.stem.2014.07.011.

Courtney DG, Atkinson SD, Allen EH, Moore JE, Walsh CP, Leslie Pedrioli DM, Macewen CJ, Pellegrini G, Maurizi E, Serafini C, Fantacci M, Liao H, Irvine AD, McLean WH, Moore TC. siRNA Silencing of the Mutant Keratin 12 Allele in Corneal Limbal Epithelial Cells Grown from Patients with Meesmann’s Epithelial Corneal Dystrophy. Invest Ophthalmol Vis Sci. 2014 May 6;55(5):3352-60. doi: 10.1167/iovs.13-12957.

Bianco P, Cattaneo E, De Luca M, Pani L. Stamina therapies: Let the record stand. Nature. 2014 Feb 27;506(7489):434. doi: 10.1038/506434c.

Courtney DG, Atkinson SD, Moore JE, Maurizi E, Serafini C, Pellegrini G, Black GC, Manson F, Yam GH, Allen EH, McLean WH, Moore TC. Development of allele specific gene silencing siRNAs for TGFBI Arg124Cys in Lattice Corneal Dystrophy Type I. Invest Ophthalmol Vis Sci. 2014 Feb 18;55(2):977-85. doi: 10.1167/iovs.13-13279

Pellegrini G, Rama P, Di Rocco A, Panaras A, De Luca M. Concise review: hurdles in a successful example of limbal stem cell-based regenerative medicine. Stem Cells. 2014 Jan;32(1):26-34. doi: 10.1002/stem.1517.

De Rosa L, Carulli S,  Cocchiarella F, Quaglino D, Enzo E, Franchini E, Giannetti A, De Santis G, Recchia A, Pellegrini G, De Luca M. Long-term stability and safety of transgenic cultured epidermal stem cells in gene therapy of junctional epidermolysis bullosa. Stem Cell Reports. 2014 Jan 14;2(1):1-8. doi: 10.1016/j.stemcr.2013.11.001. eCollection 2014.

2013

Pellegrini G, Rama P, Matuska S, Lambiase A, Bonini B, Pocobelli A, Gisoldi Colabelli R, Spadea L, Fasciani R, Ballestrazzi E, Vinciguerra P, Rosetta P, Tortori A, Nardi M, Gabbriellini G, Traverso CE., Macaluso C, Losi L, Percesepe A, Venturi B, Corradini F, Panaras A, Di Rocco A, Guatelli P, De Luca M. Biological parameters determining the clinical outcome of cultured limbal stem cells. Regen Med. 2013 Sep;8(5):553-67. doi: 10.2217/rme.13.43. Epub 2013 Jun 3.

Carulli S, Contin R, De Rosa L, Pellegrini G, De Luca M. The long and winding road that leads to a cure for epidermolysis bullosa. Regen Med. 2013 Jul;8(4):467-81. doi: 10.2217/rme.13.33.

Bianco P, Barker R, Brüstle O, Cattaneo E, Clevers H, Daley GQ, De Luca M, Goldstein L, Lindvall O, Mummery C, Robey PG, Sattler de Sousa E Brito C, Smith A. Regulation of stem cell therapies under attack in Europe: for whom the bell tolls. EMBO J. 2013 May 29;32(11):1489-95. doi: 10.1038/emboj.2013.114. Epub 2013 May 3.

2012

De Rosa L, De Luca M. Cell biology: Dormant and restless skin stem cells. Nature. 2012 Sep 13;489(7415):215-7. doi: 10.1038/489215a.

Corradini F, Venturi B, Pellegrini G, De Luca M. Methods for characterization/manipulation of human corneal stem cells and their applications in regenerative medicine. Methods Mol Biol. 2012;916:357-72. doi: 10.1007/978-1-61779-980-8_26.

2011

Zinzani PL, Connors JM, Knight AD, Crowe AM, Jones RA, Tucker B. Analysing the benefits and cost savings of oral vs iv fludarabine for management of B-Cell Chronic Lymphocytic Leukemia (CLL). Value in Health. 2011 May; 14(3):A63

Pellegrini G, Rama P, De Luca M. Vision from the right stem. Trends Mol Med. 2011 Jan;17(1):1-7. Epub 2010 Nov 11

Saghizadeh M, Soleymani S, Harounian A, Bhakta B, Troyanovsky SM, Brunken WJ, Pellegrini G, Ljubimov AV . Alterations of epithelial stem cell marker patterns in human diabetic corneas and effects of c-met gene therapy. Mol Vis. 2011;17:2177-90. Epub 2011 Aug 12.

2010

Builles N, Janin-Manificat H, Malbouyres M, Justin V, Rovère MR, Pellegrini G, Torbet J, Hulmes DJ, Burillon C, Damour O, Ruggiero F. Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal reconstruction and regeneration. Biomaterials. 2010 Nov;31(32):8313-22. doi: 10.1016/j.biomaterials.2010.07.066. Epub 2010 Aug 13.

Rama P, Matuska S, Paganoni G, Spinelli A. De Luca M, Pellegrini G. Limbal stem-cell therapy and long-term corneal regeneration. N Engl J Med. 2010 Jul 8;363(2):147-55. doi: 10.1056/NEJMoa0905955. Epub 2010 Jun 23.

Colabelli Gisoldi RA, Pocobelli A, Villani CM, Amato D, Pellegrini G. Evaluation of molecular markers in corneal regeneration by means of autologous cultures of limbal cells and keratoplasty. Cornea. 2010 Jul;29(7):715-22. doi: 10.1097/ICO.0b013e3181c91ac4.

Pellegrini G, De Luca M. Human embryonic stem-cell derived keratinocytes: how close to clinics?. Cell Stem Cell. 2010 Jan 8;6(1):8-9. doi: 10.1016/j.stem.2009.12.002.

2009

Lambiase A, Micera A, Pellegrini G, Merlo D, Rama P, De Luca M, Bonini S, Bonini S. In vitro evidence of nerve growth factor effects on human conjunctival epithelial cell differentiation and mucin gene expression. Invest Ophthalmol Vis Sci. 2009 Oct;50(10):4622-30. doi: 10.1167/iovs.08-2716. Epub 2009 Apr 30.

De Luca M, Pellegrini G, Mavilio F. Gene therapy of inherited skin adhesion disorders: A critical overview. Br J Dermatol. 2009 Jul;161(1):19-24. doi: 10.1111/j.1365-2133.2009.09243.x. Epub 2009 Apr 20.

Ahrlund-Richter L, De Luca M, Marshak DR, Munsie M, Veiga A, Rao M, Isolation and production of cells suitable for human therapy: challenges ahead. Cell Stem Cell. 2009 Jan 9;4(1):20-6. doi: 10.1016/j.stem.2008.11.012. Epub 2008 Dec 8.

Pellegrini G, Rama P, Mavilio F, De Luca M. Epithelial stem cells in corneal regeneration and epidermal gene therapy. J Pathol. 2009 Jan;217(2):217-28. doi: 10.1002/path.2441.

2008

Hyun I, Lindvall O, Ahrlund-Richter L, Cattaneo E, Cavazzana-Calvo M, Cossu G, De Luca M, Fox IJ, Gerstle C, Goldstein RA, Hermerén G, High KA, Kim HO, Lee HP, Levy- Lahad E, Li L, Lo B, Marshak DR, McNab A, Munsie M, Nakauchi H, Rao M, Rooke HM, Valles CS, Srivastava A, Sugarman J, Taylor PL, Veiga A, Wong AL, Zoloth L, Daley GQ. New ISSCR Guidelines Underscore Major Principles for Responsible Translational Stem Cell Research. Cell Stem Cell. 2008 Dec 4;3(6):607-9. doi: 10.1016/j.stem.2008.11.009.

De Luca M, Pellegrini G, Mavilio F. Gene therapy of inherited skin adhesion disorders. Drug Discovery Today: Therapeutic Strategies. 2008 Dec;5(4):249-254. Doi: 10.1016/j.ddstr.2009.04.001.

Di Nunzio F, Maruggi G, Ferrari S, Di Iorio E, Poletti V, Garcia M, Del Rio M, De Luca M, Larcher F, Pellegrini G, Mavilio F. Correction of laminin-5 deficiency in human epidermal stem cells by transcriptionally targeted lentiviral vectors. Mol Ther. 2008 Dec;16(12):1977-85. doi: 10.1038/mt.2008.204. Epub 2008 Sep 23.

Vrana NE, Builles N, Justin V, Bednarz J, Pellegrini G, Ferrari B, Damour O, Hulmes DJ, Hasirci V. Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures. Invest Ophthalmol Vis Sci. 2008 Dec;49(12):5325-31. doi: 10.1167/iovs.07-1599. Epub 2008 Aug 15.

Vinciguerra P, Albè E, Rosetta P, Di Iorio E, Pellegrini G. Custom phototherapeutic keratectomy and autologous fibrin-cultured limbal stem cell autografting: a combined approach. J Refract Surg. 2008 Apr;24(4):323-4.

Pubblicazioni precedenti di rilievo

2007

Pellegrini G, De Luca M, Arsenijevic Y. Towards therapeutic application of ocular stem cells. Semin Cell Dev Biol. 2007 Dec;18(6):805-18. Epub 2007 Sep 16.

Barbaro V, Testa A, Di Iorio E, Mavilio F, Pellegrini G, De Luca M . C/EBPd regulates cell cycle and self- renewal of human limbal stem cells. J Cell Biol. 2007 Jun 18;177(6):1037-49. Epub 2007 Jun 11.

2006

Mavilio F, Pellegrini G, Ferrari S, Di Nunzio F, Di Iorio E, Recchia A, Maruggi G, Ferrari G, Bonini C, Capurro S, Conti A, Magnoni C, Giannetti A, De Luca M. Correction of junctional epidermolysis bullosa by transplantation of genetically modified epidermal stem cells. Nat Med. 2006 Dec;12(12):1397-402. Epub 2006 Nov 19.

Barbaro V, Di Iorio E, Ferrari S, Bisceglia L, Ruzza A, De Luca M, Pellegrini G. Expression of VSX1 in human corneal keratocytes during differentiation to myofibroblasts in response to wound healing. Invest Ophthalmol Vis Sci. 2006 Dec;47(12):5243-50.

Di Iorio E, Barbaro V, Ferrari S, Ortolani C, De Luca M, Pellegrini G. Q-FIHC: quantification of fluorescence immunohistochemistry to analyse p63 isoforms and cell cycle phases in human limbal stem cells. Microsc Res Tech. 2006 Dec;69(12):983-91.

Vetrini F, Tammaro R, Bondanza S, Surace EM, Auricchio A, De Luca M, Ballabio A, Marigo V. Aberrant splicing in the ocular albinism type 1 gene (OA1/GPR143) is corrected in vitro by morpholino antisense oligonucleotides. Hum Mutat. 2006 May;27(5):420-6.

Ferrari S, Pellegrini G, Matsui T, Mavilio F, De Luca M. Towards a gene therapy clinical trial for epidermolysis bullosa. Rev Recent Clin Trials. 2006 May;1(2):155-62.

Ferrari S, Pellegrini G, Matsui T, Mavilio F, De Luca M. Gene therapy in combination with tissue engineering to treat epidermolysis bullosa. Expert Opin Biol Ther. 2006 Apr;6(4):367-78.

De Luca M, Pellegrini G, Green H. Regeneration of squamous epithelia from stem cells of cultured grafts. Regen Med. 2006 Jan;1(1):45-57.

2005

Volpi N, Maccari F, Ferrari S, De Luca M, Pellegrini G. Separation of keratan-sulfate-derived disaccharides by high-performance liquid chromatography and postcolumn derivatization with 2-cyanoacetamide and fluorimetric detection. Anal Biochem. 2005 Jul 15;342(2):200-5.

Di Iorio E, Barbaro V, Ruzza A, Ponzin D, Pellegrini G, De Luca M. Isoforms of DNp63 and the migration of ocular limbal cells in human corneal regeneration. Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9523-8. Epub 2005 Jun 27.

Ferrari S, Pellegrini G, Mavilio F, De Luca M. Gene therapy approaches for epidermolysis bullosa. Clin Dermatol. 2005 Jul-Aug;23(4):430-6.

Lemoli RM, Bertolini F, Cancedda R, De Luca M, Del Santo A, Ferrari G, Ferrari S, Martino G, Mavilio F, Tura S. Stem cell plasticity: time for a reappraisal? Haematologica. 2005 Mar;90(3):360-81.

2004

Pellegrini G, Dellambra E, Paterna P, Golisano O, Traverso CE, Rama P, Lacal P, De Luca M. Telomerase activity is sufficient to bypass replicative senescence in human limbal and conjunctival but not corneal keratinocytes. Eur J Cell Biol. 2004 Dec;83(11-12):691-700.

Pellegrini G. Changing the cell source in cell therapy?.  N Engl J Med. 2004 Sep 16;351(12):1170-2.

Guerra L, Primavera G, Raskovic D, Pellegrini G, Golisano O, Bondanza S, Kuhn S, Piazza P, Luci A, Atzori F, De Luca M. Permanent repigmentation of piebaldism by erbium:YAG laser and autologous cultured epidermis. Br J Dermatol. 2004 Apr;150(4):715-21.

Abbruzzese C, Kuhn U, Molina F, Rama P, De Luca M. Novel mutations in the CHST6 gene causing macular corneal dystrophy. Clin Genet. 2004 Feb;65(2):120-5.

2003

Guerra L, Primavera G, Raskovic D, Pellegrini G, Golisano O, Bondanza S, Paterna P, Sonego G, Gobello T, Atzori F, Piazza P, Luci A, De Luca M. Erbium:YAG laser and cultured epidermis in the surgical therapy of stable vitiligo. Arch Dermatol. 2003 Oct;139(10):1303-10.

Quast T, Wehner S, Kirfel G, Jaeger K, De Luca M, Herzog V. sAPP as a regulator of dendrite motility and melanin release in epidermal melanocytes and melanoma cells. FASEB J. 2003 Sep;17(12):1739-41. Epub 2003 Jul 3.

2002

Rheinwald JG, Hahn WC, Ramsey MR, Wu JY, Guo Z, Tsao H, De Luca M, Catricalà C, O’Toole KM. A two-stage, p16(INK4A)- and p53-dependent keratinocyte senescence mechanism that limits replicative potential independent of telomere status. Mol Cell Biol. 2002 Jul;22(14):5157-72.

Schiaffino MV, Dellambra E, Cortese K, Baschirotto C, Bondanza S, Clementi M, Nucci P, Ballabio A, Tacchetti C, De Luca M. Effective retrovirus-mediated gene transfer in normal and mutant human melanocytes. Hum Gene Ther. 2002 May 20;13(8):947-57.

2001

Rama P, Bonini S, Lambiase A, Golisano O, Paterna P, De Luca M, Pellegrini G. Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency. Transplantation. 2001 Nov 15;72(9):1478-85.

Dellambra E, Prislei S, Salvati AL, Madeddu ML, Golisano O, Siviero E, Bondanza S, Cicuzza S, Orecchia A, Giancotti FG, Zambruno G, De Luca M. Gene correction of integrin beta4-dependent pyloric atresia-junctional epidermolysis bullosa keratinocytes establishes a role for beta4 tyrosines 1422 and 1440 in hemidesmosome assembly. J Biol Chem. 2001 Nov 2;276(44):41336-42. Epub 2001 Aug 24.

Pellegrini G, Dellambra E, Golisano O, Martinelli E, Fantozzi I, Bondanza S, Ponzin D, McKeon F, De Luca M. p63 identifies keratinocyte stem cells. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3156-61.

2000

D’Addio M, Pizzigoni A, Bassi MT, Baschirotto C, Valetti C, Incerti B, Clementi M, De Luca M, Ballabio A, Schiaffino MV. Defective intracellular transport and processing of OA1 is a major cause of ocular albinism type 1. Hum Mol Genet. 2000 Dec 12;9(20):3011-8.

Dellambra E, Pellegrini G, Guerra L, Ferrari G, Zambruno G, Mavilio F, De Luca M. Toward epidermal stem cell-mediated ex vivo gene therapy of junctional epidermolysis bullosa. Hum Gene Ther. 2000 Nov 1;11(16):2283-7.

Taichman LB, Khavari PA, De Luca M. Introductory remarks. Hum Gene Ther. 2000 Nov 1;11(16):2245.

Guerra L, Capurro S, Melchi F, Primavera G, Bondanza S, Cancedda R, Luci A, De Luca M, Pellegrini G. Treatment of “stable” vitiligo by Timedsurgery and transplantation of cultured epidermal autografts. Arch Dermatol. 2000 Nov;136(11):1380-9.

Dellambra E, Golisano O, Bondanza S, Siviero E, Molinari M, Lacal P, D’Atri S, De Luca M. Downregulation of 14-3-3s prevents clonal evolution and leads to immortalization of primary human keratinocytes. J Cell Biol. 2000 May 29;149(5):1117-30.

Foehr ED, Tatavos A, Tanabe E, Raffioni S, Goetz S, Dimarco E, De Luca M, Bradshaw RA. Discoidin domain receptor 1 (DDR1) signaling in PC12 cells: activation of juxtamembrane domains in PDGFR/DDR/TrkA chimeric receptors. FASEB J. 2000 May;14(7):973-81.

1999

Pellegrini G, Ranno R, Stracuzzi G, Bondanza S, Guerra L, Zambruno G, Micali G, De Luca M. The control of epidermal stem cells (holoclones) in the treatment of massive full-thickness burns with autologous keratinocytes cultured on fibrin. Transplantation. 1999 Sep 27;68(6):868-79.

Chistolini P, De Angelis G, De Luca M, Pellegrini G, Ruspantini I. Analysis of the mechanical properties of in vitro reconstructed epidermis: preliminary results. Med Biol Eng Comput. 1999 Sep;37(5):670-2.

Schiaffino MV, d’Addio M, Alloni A, Baschirotto C, Valetti C, Cortese K, Puri C, Bassi MT, Colla C, De Luca M, Tacchetti C, Ballabio A. Ocular albinism: evidence for a defect in an intracellular signal transduction system. Nat Genet. 1999 Sep;23(1):108-12.

Pellegrini G, Golisano O, Paterna P, Lambiase A, Bonini S, Rama P, De Luca M. Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J Cell Biol. 1999 May 17;145(4):769-82.

1998

Pellegrini G, Bondanza S, Guerra L, De Luca M. Cultivation of human keratinocyte stem cells: current and future clinical applications. Med Biol Eng Comput. 1998 Nov;36(6):778-90.

Vailly J, Gagnoux-Palacios L, Dell’Ambra E, Roméro C, Pinola M, Zambruno G, De Luca M, Ortonne JP, Meneguzzi G. Corrective gene transfer of keratinocytes from patients with junctional epidermolysis bullosa restores assembly of hemidesmosomes in reconstructed epithelia. Gene Ther. 1998 Oct;5(10):1322-32.

Dellambra E, Vailly J, Pellegrini G, Bondanza S, Golisano O, Macchia C, Zambruno G, Meneguzzi G, De Luca M. Corrective transduction of human epidermal stem cells in laminin-5-dependent junctional epidermolysis bullosa. Hum Gene Ther. 1998 Jun 10;9(9):1359-70.

Bellini MH, Mathor MB, De Luca M, Cancedda R, Bartolini P. Ultrasensitive in vivo bioassay detects bioactive human growth hormone in transduced primary human keratinocytes. J Endocrinol Invest. 1998 Jan;21(1):1-6.

1997

Marchisio PC, Trusolino L, De Luca M. Topography and biological role of integrins in human skin. Microsc Res Tech. 1997 Aug 15;38(4):353-60.

Malorni W, Guiducci G, Pasquinelli G, Rivabene R, Re RC., Ramazzotti E, De Luca M, La Placa M, Cenacchi G. HIV-type 1 induces specific cytoskeleton alterations in human epithelial cells in culture. Eur. J. Dermatol. 1997 Jun;7(4): 263-269.

De Luca M, Pellegrini G. The importance of epidermal stem cells in keratinocyte-mediated gene therapy. Gene Ther. 1997 May;4(5):381-3.

Tsubota K, De Luca M, Pellegrini G. Corneal epithelial stem-cell transplantation. Lancet. 1997 May 24; 349:1556.

Pellegrini G, Traverso C, Franzi AT, Zingirian M, Cancedda R, De Luca M. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 1997 Apr 5;349(9057):990-3.

1996

Mathor MB, Ferrari G, Dellambra E, Cilli M, Mavilio F, Cancedda R, De Luca M. Clonal analysis of stably transduced human epidermal stem cells in culture. Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10371-6.

Schiaffino MV, Baschirotto C, Pellegrini G, Montalti S, Tacchetti C, De Luca M, Ballabio A. The ocular albinism type 1 gene product is a membrane glycoprotein localized to melanosomes. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9055-60.

Cenacchi G, Guiducci G, Pasquinelli G, Re MC, Ramazzotti E, Furlini G, Malorni W, De Luca M, Martinelli GN.  Early ultrastuctural changes of human keratinocytes after HIV-1 contact: an in vitro study. Eur. J. Dermatol. 1996;6(3): 213-218.

1995

Dellambra E, Patrone M, Sparatore B, Negri A, Ceciliani F, Bondanza S, Molina F, Cancedda FD, De Luca M. Stratifin, a keratinocyte specific 14-3-3 protein, harbors a pleckstrin homology (PH) domain and enhances protein kinase C activity. J Cell Sci. 1995 Nov;108 ( Pt 11):3569-79.

Ramarli D, Giri A, Reina S, Poffe O, Cancedda R, Varnier O, Tridente G, De Luca M. HIV-1 spreads from lymphocytes to normal human keratinocytes suitable for autologous and allogenic transplantation. J Invest Dermatol. 1995 Nov;105(5):644-7.

Zambruno G, Marchisio PC, Marconi A, Vaschieri C, Melchiori A, Giannetti A, De Luca M. Transforming growth factor-b1 modulates b1 and b5 integrin receptors and induces the de novo expression of the avb6 heterodimer in normal human keratinocytes: implications for wound healing. J Cell Biol. 1995 May;129(3):853-65.

1994

De Luca M, Pellegrini G, Zambruno G, Marchisio PC. Role of integrins in cell adhesion and polarity in normal keratinocytes and human skin pathologies. J Dermatol. 1994 Nov;21(11):821-8.

Giannelli G, Savoia P, Schiraldi O, Lospalluti M, De Luca M, Marchisio PC, Quaranta V. Psoriatic lesions in patients with chronic liver disease are distinct from psoriasis vulgaris lesions, as judged on basis of integrin adhesion receptors. Hepatology. 1994 Jul;20(1 Pt 1):56-65.

De Luca M. Keratinocyte-melanocyte interactions in in vitro reconstituted human epidermis. Chron. Derm. 1994;IV:6, 935-940.

1993

Di Marco E, Cutuli N, Guerra L, Cancedda R, De Luca M. Molecular cloning of trkE, a novel trk-related putative tyrosine kinase receptor isolated from normal human keratinocytes and widely expressed by normal human tissues. J Biol Chem. 1993 Nov 15;268(32):24290-5.

Di Marco E, Mathor M, Bondanza S, Cutuli N, Marchisio PC, Cancedda R, De Luca M. Nerve growth factor binds to normal human keratinocytes through high and low affinity receptors and stimulates their growth by a novel autocrine loop. J Biol Chem. 1993 Oct 25;268(30):22838-46.

Romagnoli G, De Luca M, Faranda F, Franzi AT, Cancedda R. One-step treatment of posterior hypospadias by the autologous graft of cultured urethral epithelium. J Urol. 1993 Oct;150(4):1204-7.

De Luca M, Siegrist W, Bondanza S, Mathor M, Cancedda R, Eberle AN. Alpha melanocyte stimulating hormone (alpha MSH) stimulates normal human melanocyte growth by binding to high-affinity receptors. J Cell Sci. 1993 Aug;105 ( Pt 4):1079-84.

Zambruno G, Marchisio PC, Melchiori A, Bondanza S, Cancedda R, De Luca M. Expression of integrin receptors and their role in adhesion, spreading and migration of normal human melanocytes. J Cell Sci. 1993 May;105 ( Pt 1):179-90.

Marchisio PC, Cremona O, Savoia P, Pellegrini G, Ortonne JP, Verrando P, Burgeson RE, Cancedda R, De Luca M. The basement membrane protein BM-600/nicein codistributes with kalinin and the integrin alpha 6 beta 4 in human cultured keratinocytes. Exp Cell Res. 1993 Apr;205(2):205-12.

Cancedda R, De Luca M. Tissue engineering for clinical application. Year Immunol. 1993;7:193-8.

1992

Rugolo M, Mastrocola T, De Luca M, Romeo G, Galietta LJ. A volume-sensitive chloride conductance revealed in cultured human keratinocytes by 36Cl- efflux and whole-cell patch clamp recording. Biochim Biophys Acta. 1992 Nov 23;1112(1):39-44.

De Luca M, Pellegrini G, Bondanza S, Cremona O, Savoia P, Cancedda R, Marchisio PC. The control of polarized integrin topography and the organization of adhesion-related cytoskeleton in normal human keratinocytes depend upon number of passages in culture and ionic environment. Exp Cell Res. 1992 Sep;202(1):142-50.

Pellegrini G, De Luca M, Orecchia G, Balzac F, Cremona O, Savoia P, Cancedda R., Marchisio PC, Expression, topography and function of integrin receptors is severely altered in keratinocytes from involved and uninvolved psoriatic skin. J Clin Invest. 1992 Jun;89(6):1783-95.

Barreca A, De Luca M, Del Monte P, Bondanza S, Damonte G, Cariola G, Di Marco E, Giordano G, Cancedda R, Minuto F. In vitro paracrine regulation of human keratinocyte growth by fibroblast-derived insulin-like growth factors. J Cell Physiol. 1992 May;151(2):262-8.

De Luca M, Albanese E, Cancedda R, Viacava A, Faggioni A, Zambruno G, Giannetti A. Treatment of leg ulcers with cryopreserved allogeneic cultured epithelium. A multicenter study. Arch Dermatol. 1992 May;128(5):633-8.

Gherzi R, Melioli G, De Luca M, D’Agostino A, Distefano G, Guastella M, D’Anna F, Franzi AT, Cancedda R. “HepG2/erythroid/brain” type glucose transporter (GLUT1) is highly expressed in human epidermis: keratinocyte differentiation affects GLUT1 levels in reconstituted epidermis. J Cell Physiol. 1992 Mar;150(3):463-74.

De Luca M, Bondanza S, Cancedda R, Tamisani AM, Di Noto C, Muller L, Dioguardi D, Brienza E, Calvario A, Zermani R, et al. Permanent coverage of full skin thickness burns with autologous cultured epidermis and reepithelialization of partial skin thickness lesions induced by allogeneic cultured epidermis: a multicentre study in the treatment of children. Burns. 1992;18 Suppl 1:S16-9.

De Luca M, Cancedda R. Culture of human epithelium. Burns. 1992;18 Suppl 1:S5-10.

Pellegrini G, Gherzi R, Franzi AT, D’Anna F, De Luca M, Cancedda R. In vitro reconstitution of differentiated human epithelia. ATLA. 1992; 20:95-102.

1991

Mastrocola T, De Luca M, Rugolo M. Characterization of chloride transport pathways in cultured human keratinocytes. Biochim Biophys Acta. 1991 Nov 21;1097(4):275-82.

Di Marco E, Marchisio PC, Bondanza S, Franzi AT, Cancedda R, De Luca M. Growth-regulated synthesis and secretion of biologically active nerve growth factor by human keratinocytes. J Biol Chem. 1991 Nov 15;266(32):21718-22.

Gherzi R, Melioli G, De Luca M, D’Agostino A, Guastella M, Traverso CE, D’Anna F, Franzi AT, Cancedda R. High expression levels of the “erythroid/brain” type glucose transporter (GLUT1) in the basal cells of human eye conjunctiva and oral mucosa reconstituted in culture. Exp Cell Res. 1991 Jul;195(1):230-6.

Galietta LJ, Barone V, De Luca M, Romeo G. Characterization of chloride and cation channels in cultured human keratinocytes. Pflugers Arch. 1991 Mar;418(1-2):18-25.

Marchisio PC, Bondanza S, Cremona O, Cancedda R, De Luca M. Polarized expression of integrin receptors (alpha 6 beta 4, alpha 2 beta 1, alpha 3 beta 1, and alpha v beta 5) and their relationship with the cytoskeleton and basement membrane matrix in cultured human keratinocytes. J. Cell Biol. 1991 Feb;112(4):761-73.

Rugolo M, De Luca M, Mastrocola T. Chloride transport pathways in human keratinocytes. Adv Exp Med Biol. 1991;290:297-304; discussion 304-5.

1990

Marchisio PC, Cancedda R, De Luca M. Structural and functional studies of integrin receptors in cultured human keratinocytes. Cell Differ Dev. 1990 Dec 2;32(3):355-9.

De Luca M, Albanese E, Megna M, Cancedda R, Mangiante PE, Cadoni A, Franzi AT. Evidence that human oral epithelium reconstituted in vitro and transplanted onto patients with defects in the oral mucosa retains properties of the original donor site. Transplantation. 1990 Sep;50(3):454-9.

De Luca M, Tamura RN, Kajiji S, Bondanza S, Rossino P, Cancedda R, Marchisio PC, Quaranta V. Polarized integrin mediates human keratinocyte adhesion to basal lamina. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6888-92.

Romagnoli G, De Luca M, Faranda F, Bandelloni R, Franzi AT, Cataliotti F, Cancedda R. Treatment of posterior hypospadias by the autologous graft of cultured urethral epithelium. N Engl J Med. 1990 Aug 23;323(8):527-30.

1989

Raiteri M, Pellegrini G, Cantoni C, Bonanno G. A novel type of GABA receptor in rat spinal cord? Naunyn Schmiedebergs Arch Pharmacol. 1989 Dec;340(6):666-70.

De Luca M, Albanese E, Bondanza S, Megna M, Ugozzoli L, Molina F, Cancedda R, Santi PL, Bormioli M, Stella M, et al. Multicentre experience in the treatment of burns with autologous and allogenic cultured epithelium, fresh or preserved in a frozen state. Burns. 1989 Oct;15(5):303-9.

Bonanno G, Pellegrini G, Asaro D, Fontana G, Raiteri M. GABAB autoreceptors in rat cortex synaptosomes: response under different depolarizing and ionic conditions. Eur J Pharmacol. 1989 Mar 7;172(1):41-9

Bonanno G, Cavazzani P, Andrioli GC, Asaro D, Pellegrini G, Raiteri M. Release-regulating autoreceptors of the GABAB-type in human cerebral cortex. Br J Pharmacol. 1989 Feb;96(2):341-6.

1988

De Luca M, D’Anna F, Bondanza S, Franzi AT, Cancedda R. Human epithelial cells induce human melanocyte growth in vitro but only skin keratinocytes regulate its proper differentiation in the absence of dermis. J Cell Biol. 1988 Nov;107(5):1919-26.

De Luca M, Franzi AT, D’Anna F, Zicca A, Albanese E, Bondanza S, Cancedda R. Coculture of human keratinocytes and melanocytes: differentiated melanocytes are physiologically organized in the basal layer of the cultured epithelium. Eur J Cell Biol. 1988 Apr;46(1):176-80.

D’Anna F, De Luca M, Cancedda R, Zicca A, Franzi AT. Elutriation of human keratinocytes and melanocytes from in vitro cultured epithelium. Histochem. J. (J. Mol. Histol.) 1988;20:674-678.

1987

Pittaluga A, Asaro D, Pellegrini G, Raiteri M. Studies on [3H]GABA and endogenous GABA release in rat cerebral cortex suggest the presence of autoreceptors of the GABAB type. Eur J Pharmacol. 1987 Nov 24;144(1):45-52.

Consiglio E. Acquaviva AM, Formisano S, Liguoro D, Gallo A, Vittorio T, Santisteban P, De Luca M, Shifrin S, Yeh HJ et al. Characterization of phosphate residues on thyroglobulin. J Biol Chem. 1987 Jul 25;262(21):10304-14.

Franzi AT, D’Anna F, Zoli S, Albanese E, De Luca M, Cancedda R. [Separation, by elutriation, of human keratinocytes cultured in vitro – article in Italian]. Boll Soc Ital Biol Sper. 1987 May 31;63(5):391-7.

Chan J, Santisteban P, De Luca M, Isozaki O, Grollman E, Kohn L. TSH receptor structure. Acta Endocrinol Suppl (Copenh). 1987;281:166-72.

1986

Kohn LD, Alvarez F, Marcocci C, Kohn AD, Corda D, Hoffman WE, Tombaccini D, Valente WA, De Luca M, Santisteban P, et al. Monoclonal antibody studies defining the origin and properties of autoantibodies in Graves’ disease. Ann N Y Acad Sci. 1986;475:157-73.

Kohn LD, Chan J, Alvarez F, Corda D, Hoffman WE, Kohn AD, Rotella C, Isosaki O, Santisteban P, De Luca M, Grollman EF. Mono-clonal Antibodies to the Thyrotropin (TSH) Receptor in the study of Graves’ Disease: Idiotypes and Antiidiotypes. Clinical Immunology Newsletter. 1986; 7:182-185.

1984

Monaco F, Carducci C, De Luca M, Andreoli M, Dominici R. Human undifferentiated thyroid carcinoma synthesizes and secretes 19S thyroglobulin. Cancer. 1984 Jul 1;54(1):79-83.

1983

Rotella CM, Tanini A, Consiglio E, Shifrin S, De Luca M, Toccafondi R, Kohn LD. Specificity of thyroglobulin interactions with thyroid cells and membranes. Biochem Biophys Res Commun. 1983 Aug 12;114(3):962-8.

1982

Monaco F, De Luca M, Carducci C, Pontecorvi A, D’Armiento M, Roche J. Sur l’inhibition de la biosynthese de la thyroglobuline dans le corps thyroide du rat par l’isoniazide. C R Seances Soc Biol Fil. 1982;176(5):613-8.

Monaco F, Pontecorvi A, De Luca M, De Pirro R, D’Armiento M, Roche J. Inhibition de la biosynthese de la thyroglobuline par le propranolol chez le rat. C R Seances Soc Biol Fil. 1982;176(5):607-12.

L’azienda

Holostem Terapie Avanzate è uno spin-off universitario nato nel 2008 dal prezioso connubio tra gli scienziati Michele De Luca e Graziella Pellegrini, l’Università degli Studi di Modena e Reggio Emilia e Chiesi Farmaceutici S.p.A.

leggi di più

Management

Holostem vanta un management team di grande prestigio, che apporta alla società un solido know-how sia nel mondo della gestione aziendale sia in quello della ricerca accademica, applicata con successo nella medicina traslazionale.

leggi di più

Background scientifico

Michele De Luca e Graziella Pellegrini sono considerati leader a livello internazionale nel campo della biologia delle cellule staminali degli epiteli di rivestimento mirata alla loro applicazione clinica in protocolli di terapie cellulare a genica.

leggi di più

Menu